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Report: Puntledge River Summer Chinook
Parentage-based Tagging Study
Year 3 FWCP COA-F17-F-1182
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If you have any questions on the information presented, or require additional report data or attachments, please contact the Report Contact
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Genetic analysis methods will be used in this multi-year study to identify individual Puntledge River summer-run Chinook salmon back to parental crosses (both those that were performed in the hatchery and those that occurred in the wild) to study the effects of parental Chinook return migration time and BKD status on their progeny.
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Author: M. Wetklo, R. Withler, E. Guimond
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Old Reference Number: COA-F17-F-1182
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Old Reference System: FWCP Fish Wildlife Compensation Program - Coastal
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Date Published: Mar 2017
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Report ID: 52591
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Audience: Government and Public
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Genetic analysis methods will be used in this multi-year study to identify individual Puntledge River summer-run Chinook salmon back to parental crosses (both those that were performed in the hatchery and those that occurred in the wild) to study the effects of parental Chinook return migration time and BKD status on their progeny. The genetic analysis is known as parentage-based tagging and it allows identification of an individual offspring (at any age, including adults) to its parental pair, as long as both parents have been sampled and genotyped. The genotyping of parents and offspring will be conducted with a set of fifteen microsatellite loci (genetic markers) that are analyzed in the Molecular Genetics lab (MGL) at the Pacific Biological Station. Fisheries and Oceans Canada (DFO) considers the Puntledge River summer-run Chinook salmon a population of high conservation concern. This research will provide information on the most effective strategies to implement in re-establishing successful reproduction both in the hatchery and in the wild. This project is identified under the Species Based Actions in Table 2 of the Salmonid Action Plan Support hatchery activities focused on enhancing the earliest returning adults of the summer Chinook run - which is ranked as a Level 1 priority.
To date, high quality DNA extracted from adult summer Chinook salmon sampled from the Puntledge River in 2013 - 2015 has been analyzed. Tissue samples from 211 brood year 2015 summer Chinook adults sampled from the natural environment above the Puntledge diversion dam, the principle summer Chinook spawning grounds, and 702 naturally-spawned juveniles collected between February and July 2016 during outmigration were successfully analyzed. The 211 natural spawners transported to Comox Lake were estimated to be approximately 59% of the Summer Chinook natural spawners that emigrated past the hatchery brood collection point in the lower river during 2015.
DNA extraction and analysis of brood year 2016 hatchery and natural spawners is underway in the Molecular Genetics Lab at the Pacific Biological Station. Also being analyzed are 147 and 700 jack samples collected in the adult returns of 2015 and 2016. A subset of these returns are believed to be progeny from the 2013 and 2014 hatchery brood fish and natural spawners. The genotypes from the jack samples will be used to test our ability to assign returning progeny back to a unique pair of parents in our spawner DNA database.
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Report Type
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Fish and Aquatic Habitat Information |
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Subject
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Fish Species - Chinook Salmon - Oncorhynchus tshawytscha |
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Region - Lower Mainland |
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Fish and Fish Habitat - Conservation Biology |
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Fish and Fish Habitat - Fish Genetics |
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