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The effect of scion collection and storage on graft survival of lodgepole pine BC Forest Service - Research Division
1977
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Abstract: Nearly 5,000 grafts representing 150 clones are produced annually to meet the establishment requirements of the Lodgepole Pine Improvement Program's clonal breeding orchards. Considerable time, effort and money are expended in the selection of parent-trees in natural stands and the subsequent collection of scion materials from those trees for the purpose stated above. Considering the substantial investment made in the propagation phase of this program, good graft survival is essential to minimize costs. This memo summarizes the results of trials designed to determine optimal scion collection and storage parameters to ensure good graft survival of lodgepole pine in central B.C. Date of Scion Collection - Collection of scion material is often hampered by inaccessibility in the interior (e.g. snow in Winter, washouts in Spring, etc.). To determine how early material could be gathered in the Fall prior to heavy snowfall, scions were collected from 6 selections around the first day of the month, from October to December. Survival based on date of collection was 42, 58 and 75% for October, November and December, respectively. Figures based on very large samples (>500) indicated collection after December 1 and before April 15 insured excellent graft survival. Survival rates for the months of October, February, March and April were 78, 87, 92 and 88%, respectively. These materials were not clonally replicated by month. Temperature and Duration of Scion Storage - Material was collected from each of 5 clones in mid-December and stored at 2 temperatures for periods of 1, 3 and 6 months prior to grafting. Differences in graft survival as affected by the storage temperatures, -2C and -15C, were very slight. Temperatures above freezing are used for storing materials for very brief periods only. Average survival over time decreased from 94% after one month to 84% after 6 months in storage. Clonal response to duration of storage in terms of graft survival did exist but was relatively insignificant. Recommendation - Scion material for lodegpole pine should be collected no earlier than October 15 and preferably after December 1st to insure reasonable survival and good vigor of grafted materials. Scions should be stored in air-tight, moisture-tight containers or bags at sub-freezing temperatures for periods not exceeding 6-7 months. Material collected prior to complete dormancy (Oct. - Nov.) should be grafted within 3-4 months of the date of collection. Requests for further information regarding the subject of this memo should be addressed to N. Wheeler, Ministry of Forests, Red Rock Research Centre, R.R. #7, 15 Mile Road, Prince George, B.C. V2N 2J5. [Abstract contains full text of memo.]
 
BC Forest Service - Research Division. 1977. The effect of scion collection and storage on graft survival of lodgepole pine. British Columbia Ministry of Forests, Lands, Natural Resource Operations and Rural Development. Research Memo (FLNRORD). RM23
 
Topic: FLNRORD Research Program
Keywords: FLNRORD, Research Memo, British Columbia
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