This project has achieved two objectives:
1) Species identification of fungal samples. DNA was extracted from fungal samples obtained from the Heningram Lake trial, and the D1-D2 region of ribosomal DNA was amplified from these samples and sequenced. The species of the sample was then identified by searching the NCBI database with the sequence obtained from its DNA.
2) Design PCR Primers which are specific for the DNA from Armillaria ostoyae and Armillaria sinapina. To design these primers, DNA was isolated from A. ostoyea and A. sinapina samples obtained from root disease infection centers in the Pinnell Road and Gavin Lake areas. DNA from the intergenic spacer of the ribosomal DNA region was amplified and sequenced. A comparison of these sequences with those from the NCBI database was used to design PCR primers that are specific for A. ostoyae or A. sinapina, and these were tested on extracted DNA samples to evaluate their potential as an identification test.