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Report: Puntledge River Summer Chinook Parentage-based Tagging Study Year 5 FWCP COA-F19-F-2697

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Tissue sampling of Puntledge summer Chinook for this study began in 2013 with the collection of hatchery broodstock, and in subsequent years (2014 to 2018) both the hatchery brood stock and potential spawners in the natural environment were sampled. In total, 2889 samples were successfully genotyped and included in the genetic analysis. In both 2017 and 2018, about 85% of the sampled adult returns were assigned to hatchery parents and therefore hatchery-origin.

Author:  Withler, R.E., J. Supernault, M. Wetklo, E. Guimond

Old Reference Number:  COA-F19-F-2697

Old Reference System:  FWCP Fish Wildlife Compensation Program - Coastal

Date Published:  Mar 2019

Report ID:  57515

Audience:  Government and Public

Genetic analysis methods were used in a multi-year study to identify individual Puntledge River summer-run Chinook salmon back to parental crosses (both those that were performed in the hatchery and those that occurred in the wild) to study the effects of parental Chinook return migration time and bacterial kidney disease (BKD) status on their progeny. The genetic analysis is known as parentage-based tagging and allows identification of an individual offspring (at any age, including adults) to its parental pair, as long as both parents have been sampled and genotyped. The genotyping of parents and offspring were conducted with a set of fifteen microsatellite loci (genetic markers) that are analyzed in the Molecular Genetics lab (MGL) at the Pacific Biological Station. Fisheries and Oceans Canada (DFO) considers the Puntledge River summer-run Chinook salmon a population of high conservation concern. This research will provide information on the most effective strategies to implement in re-establishing successful reproduction both in the hatchery and in the wild. This project is identified in the Puntledge River Watershed Action Plan (FWCP 2017) as a Level 1 priority Research & Information Acquisition action (Action Table short description PUN.RLR.RI.13.01 Conduct DNA analyses Chinook & Steelhead-P1).

Report Type
  Fish and Aquatic Habitat Information
  Fish Species - Chinook Salmon - Oncorhynchus tshawytscha
  Region - Lower Mainland
  Fish and Fish Habitat - Conservation Biology
  Fish and Fish Habitat - Fish Genetics
  Fish and Fish Habitat - Restoration

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