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Collection of seed samples were made from reforestation seedlots representing eleven provenances from each of the coastal and interior forms of Douglas-fir. Sampling was stratified and random to characterize geographic differences. More than 200 individual seeds per sample seedlot were scored for Malate Dehydrogenase-4 (MDH-4) allozyme frequencies utilizing cellulose acetate gel electrophoresis and·histochemical stains. The data revealed distinct MDH-4 allozyme frequencies characteristic of the coastal and interior forms of Douglas fir (table below). While the allozymes MDH-4 (100) and MDH-4 (95) were equally common in the interior provenances, the MDH-4 (100) allozyme predominated in the coastal forms.
[table]
In conclusion, this biochemical method which can be applied to seedlots and which consumes very little time, permits studies of geographic variation in natural populations and might be the practical solution for the difficult question of seed origin certification.
For further information write to Dr. Francis C. Yeh, Research Division, Ministry of Forests, Parliament Buildings, Victoria, B.C. V8W 3E7.
BC Forest Service - Research Division. 1978. Detecting geographic variation in coastal and interior Douglas-fir populations by biochemical method. British Columbia Ministry of Forests, Lands, Natural Resource Operations and Rural Development. Research Memo (FLNRORD). RM31
Topic: FLNRORD Research Program
Keywords: FLNRORD, Research Memo, British Columbia
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